in fasted and fed dogs were similar to those reported A nonradioisotopic method has been developed for previously for human and rat plasma (6).
the determination of all-trans-farnesyl pyrophos-All-trans-farnesyl pyrophosphate (FPP), the immediphate (FPP), the common intermediate at the branch ate precursor of squalene, is the branch point intermepoint of the biosynthesis of cholesterol and nonsterol diate in the isoprenoid metabolic pathway with the poend products, in dog and human plasma. FPP was tential to be incorporated into either sterol or nonsterol cleaved to the parent alcohol, farnesol, by the direct end products. While most of the FPP is converted into addition of alkaline phosphatase to plasma. Farnesol cholesterol and sterol-derived products, some of it is extracted from plasma was converted into a fluoresconverted into nonsterol end products such as ubiquicent derivative with 9-anthroylcyanide. After the exnone, hemeA, dolichol, and prenyl groups that are covacess reagent was removed using an NH 2 -bonded lently attached to proteins such as Ras (7, 8). Moreover, phase cartridge, the derivative was separated by a catabolites of FPP have recently been proven to play column-switching high-performance liquid chroimportant roles as signaling molecules involved in the matographic system, followed by fluorescence detecdegradation of HMG-CoA reductase (9) and in nuclear trans farnesol was purified by column chromatography 89