Oxidants can activate signaling pathways and modulate a variety of cellular activities. Their action at a molecular level involves the post-translational modification of protein thiols. We have developed a proteomic method to monitor the reduction and oxidation of protein thiols, and identify those
โฆ LIBER โฆ
Sensitive detection of the redox state of copper proteins using fluorescence
โ Scribed by Ralf Schmauder; Sharmini Alagaratnam; Chris Chan; Thomas Schmidt; Gerard W. Canters; Thijs J. Aartsma
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 323 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1432-1327
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Reactive oxygen species (ROS) have been implicated as second messengers that activate protein kinase cascades, although the means by which ROS regulate signal transduction remains unclear. In the present study, we show that interleukin 1โค (IL1โค), H 2 O 2 , and sorbitol-induced hyperosmolarity mediat