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High-sensitivity laser-induced fluorescence detection of native proteins in capillary electrophoresis

✍ Scribed by Thomas T. Lee; Edward S. Yeung


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
622 KB
Volume
595
Category
Article
ISSN
1873-3778

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Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laserinduced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 Ο« 10 Οͺ 12 M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.