Selectivity properties of sephadex gels in column chromatography of biologically important phosphate esters

✍ Scribed by Joseph Lerner; Abner I. Schepartz

• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 768 KB
• Volume: 39
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97990-7

✦ Synopsis

The selectivity properties of Sephadex gels toward various metabolic phosphates (nucleotides and coenzymes) have been examined by column chromatography using distilled water as eluent. These compounds are grouped within a range of ICa values from 0.22 to 0.39 on Sephadex G-ro."Sephadex G-15 differs from G-IO in possessing a greater affinity for phosphates. The & values are only slightly less clustered on this gel, and there. is but a negligible increase in the range of partition coefficients. Chro. matography on G-25, on the other hand, gives a relatively good spread of & values. Several ,compounds have partition coefficients greater than 1.00. Whereas phosphate affinity decreases with increasing gel density, riboflavin, a nonphosphorylated reference compound, has the highest affinity for G-IO of all substances tested. -. * The followin'g abbreviations will be used: I? = phosphrtte; d = deoxy; AMP, CMP, GMP IMP, TMP and UMP = adenosinc-, cytidine-, guanosine-, inosine-, thymidine-, and uridine-g'monophosphntcs, respectively; IDP and TDP = the corresponding diphosphates; FMN = flevin mononucleotidc.

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