✦ LIBER ✦

Ion-exchange chromatography of biologically important phosphate esters and other compounds

✍ Scribed by Kenneth C. Blanshard; Indrajit Das; Alan J. Thomas

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 864 KB
Volume: 83
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90502-4

No coin nor oath required. For personal study only.

✦ Synopsis

Borate complexed sugars, sugar phosphates, and nucleotides present in tissue extracts were separated and quantitated in 4 hr. An anion-exchange resin column and a programmed borate/acetate buffer gradient were used. Sugar residues were determined by a very sensitive orcinol/H,SO, reaction. Samples required little preparation, and recovery of standard compounds added to tissue extracts was quantitative.

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