Aqueous size-exclusion chromatography of humic acids on a sephadex gel column with diluted phosphate buffers as eluents

✍ Scribed by Sadao Mori; Masataka Hiraide; Atsushi Mizuike

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 452 KB
• Volume: 193
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86154-1

✦ Synopsis

The elution behavior of humic acids on a Sephadex gel column is sensitive to the composition, concentration, and pH of the eluent. The concentrations of the eluent in the literature are too high to obtain the correct molecular size distribution of humic acids. By reducing the concentration of phosphate buffer eluents to about a hundredth of conventional concentrations, the correct distribution is obtained. In the proposed method, 1 ml of 0.1% sample solution is introduced into a Sephadex G-50 column (2.2~cm diameter, 55 cm long) and humic acids are eluted with a 10-j M phosphate buffer solution (pH 7-9) at a flow rate of 1 ml min-I.