𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selective Labeling of Pulmonary Surfactant Protein SP-C in Organic Solution

✍ Scribed by Inés Plasencia; Antonio Cruz; José Luis López-Lacomba; Cristina Casals; Jesús Pérez-Gil

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
128 KB
Volume
296
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Pulmonary surfactant protein SP-C has been isolated from porcine lungs and treated with dansyl isothiocyanate in chloroform:methanol 2:1 (v/v) solutions, under conditions optimized to introduce a single dansyl group covalently attached to the N-terminal amine group of the protein without loss of its native thioesther-linked palmitic chains. The resulting derivative Dans-SP-C conserves the secondary structure of native SP-C as well as the ability to promote interfacial adsorption of DPPC suspensions and to affect the thermotropic behavior of DPPC bilayers. This derivative can be used to characterize lipid-protein and protein-protein interactions of a native-like SP-C in lipid/protein complexes.

📜 SIMILAR VOLUMES

Quantitation of Pulmonary Surfactant Pro
Quantitation of Pulmonary Surfactant Protein SP-B in the Absence or Presence of Phospholipids by Enzyme-Linked Immunosorbent Assay
✍ J.M. Oviedo; F. Valiño; I. Plasencia; A.G. Serrano; C. Casals; J. Pérez-Gil 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 144 KB

We have developed an enzyme-linked immunosorbent assay (ELISA) that uses polyclonal or monoclonal anti-surfactant protein SP-B antibodies to quantitate purified SP-B in chloroform/methanol and in chloroform/methanol extracts of whole pulmonary surfactant at nanogram levels. This method has been used

Origin of the prevalent SFTPB indel g.15
Origin of the prevalent SFTPB indel g.1549C > GAA (121ins2) mutation causing surfactant protein B (SP-B) deficiency
✍ Mohammed Tredano; David N. Cooper; Manfred Stuhrmann; John Christodoulou; Nadia 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 285 KB

## Abstract The __SFTPB__ gene indel g.1549C > GAA (121ins2) accounts for about 2/3 of the mutant alleles underlying complete surfactant protein B deficiency. It is unclear, however, whether its prevalence is due to recurrent mutation or a founder effect. The underlying mutational mechanism was the

17O-nmr studies of the conformational an
17O-nmr studies of the conformational and dynamic properties of enkephalins in aqueous and organic solutions using selectively labeled analogues
✍ Constantinos Sakarellos; Ioannis P. Gerothanassis; Nicolaos Birlirakis; Theodoro 📂 Article 📅 1989 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 696 KB

## Synopsis The synthesis of Leu-enkephalin selectively l70-enriched in Gly' and Gly3 is reported. The 170-nm chemical shifts of Leu5]-and [170-Gly3, Le~~l-enkephalins in H,O are almost identical and independent of the pH. Since hydrogen bonding is the dominant factor governing the chemical shifts

DNA–protein interaction in solution: NMR
DNA–protein interaction in solution: NMR studies of the 13C-site-specifically labelled Lac operator–Lac repressor complex
✍ Gérard Lancelot; Jean-Claude Maurizot; Marcel Chassignol; Nguyeng T. Thuong; Luc 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB

## Abstract Carbon‐13 and proton resonance assignments for a DNA fragment complexed with its regulatory protein were obtain with the use of a selectively ^13^C‐labelled oligonucleotide. Data obtained on the Lac operator–Lac repressor complex from the HMQC–NOESY maps of the free and complexed Lac op