✦ LIBER ✦
DNA–protein interaction in solution: NMR studies of the 13C-site-specifically labelled Lac operator–Lac repressor complex
✍ Scribed by Gérard Lancelot; Jean-Claude Maurizot; Marcel Chassignol; Nguyeng T. Thuong; Luc Chanteloup; Jean-Marie Beau
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 236 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
Abstract
Carbon‐13 and proton resonance assignments for a DNA fragment complexed with its regulatory protein were obtain with the use of a selectively ^13^C‐labelled oligonucleotide. Data obtained on the Lac operator–Lac repressor complex from the HMQC–NOESY maps of the free and complexed Lac operator demonstrate that only minor conformational modifications are induced on complexation.