## Abstract Iodinated proteins were degraded after injection into HeLa cells at firstโorder rates with halfโlives varying from three hours for the trout nonhistone chromosomal protein, HMGโT, to 60 hours for whale myoglobin. Fluoresceinatedโbovine serum albumin (flโBSA) was degraded almost twice as
Selective Degradation of Abnormal Proteins in Mammalian Tissue Culture Cells
โ Scribed by Mario R. Capecchi, Nancy E. Capecchi, Stephen H. Hughes and Geoffrey M. Wahl
- Book ID
- 123652696
- Publisher
- National Academy of Sciences
- Year
- 1974
- Tongue
- English
- Weight
- 986 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/63900
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## Abstract The experiments show that abnormal proteins are degraded faster than normal ones in HeLa cells. Among the fragmentary proteins made in the presence of puromycin, those with low molecular weight are least stable. Proteins made after incubation with 5โfluorouracil or in the presence of so
## Abstract Canavanine, an arginine analog, is incorporated into HeLa cell protein when cells are incubated in the absence of arginine, and this incorporation can result in the production of nonfunctional enzymes or abnormal proteins. The cells degrade these abnormal proteins up to three times more