Selective binding of CD4 and CD8 T-cells to antigen presenting cells for enrichment of CMV and HIV specific T-lymphocytes
β Scribed by Giuseppina Li Pira; Federico Ivaldi; Fabrizio Manca
- Book ID
- 113718063
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 637 KB
- Volume
- 376
- Category
- Article
- ISSN
- 0022-1759
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Efficient protocols to generate cytomegalovirus (CMV)-specific T cells are required for adoptive immunotherapy. Recombinant Epstein-Barr virus (EBV) vectors called mini-EBV can be used to establish permanent B cell lines in a single step, which present the CMV antigen pp65 in a constitutive manner.
Following appropriate antigen-specific stimulation, CD4 + and CD8 + T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isol