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Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion

✍ Scribed by Helmut Brosterhus; Susanne Brings; Heike Leyendeckers; Rudolf A. Manz; Stefan Miltenyi; Andreas Radbruch; Mario Assenmacher; Jürgen Schmitz

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
140 KB
Volume
29
Category
Article
ISSN
0014-2980

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✦ Synopsis

Following appropriate antigen-specific stimulation, CD4 + and CD8 + T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isolation and characterization of live antigen-specific CD4 + and CD8 + T lymphocytes. The power of this technique is demonstrated here for HLA-A0201restricted influenza matrix protein peptide 58-66-specific CD8 + cytotoxic T lymphocytes, influenza A virus-and recombinant tetanus toxin C fragment-specific Th1 cells and tetanus toxoid-specific Th2 cells.

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