𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Expression of cutaneous lymphocyte-associated antigen on human CD4+ and CD8+ Th2 cells

✍ Scribed by Mübeccel Akdis; Sven Klunker; Michael Schliz; Kurt Blaser; Cezmi A. Akdis

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
179 KB
Volume
30
Category
Article
ISSN
0014-2980

No coin nor oath required. For personal study only.

✦ Synopsis

The cutaneous lymphocyte-associated antigen (CLA) represents the homing receptor involved in selective migration of memory/effector T cells to the skin. Numerous reports demonstrated distinct CLA expression on Th1 cells. However, T cells isolated from skin lesions and CLA + T cells circulating in peripheral blood of atopic dermatitis patients expressed high IL-5 and IL-13. Accordingly, we investigated the regulation of CLA on human type 1 and type 2 T cells. CLA was induced on freshly generated Th1 and Tc1 cells only, but not on those of type 2. Anti-CD3 stimulation was sufficient to induce CLA on Th2 cells in the absence of serum in the culture medium. In serum containing medium, IL-4 inhibited CLA and related § -fucosyltransferase mRNA expression. IL-12 and/or staphylococcal enterotoxin B (SEB) stimulation up-regulated CLA expression on either Th2 and Tc2 cells. On stimulation with IL-12, CLA was expressed on the surface of bee venom phospholipase A 2specific Th1, Th2, Th0 and T regulatory 1 clones, representing non-skin-related antigenspecific T cells. In addition, CLA could be re-induced on T cells that had lost CLA expression upon resting. These results suggest that skin-selective homing is not restricted to functional and phenotypic T cell subsets.

📜 SIMILAR VOLUMES

Quantitation of CD38 activation antigen
Quantitation of CD38 activation antigen expression on CD8+ T cells in HIV-1 infection using CD4 expression on CD4+ T lymphocytes as a biological calibrator
✍ Lance E. Hultin; Jose L. Matud; Janis V. Giorgi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB 👁 2 views

For some membrane-associated antigens, the number of molecules expressed per cell carries information about the cell's differentiation and activation state. Quantitating antigen expression by flow cytometry has immediate application in monitoring CD38 expression on CD8 ؉ T cells in human immunodefic

Induction of CD69 antigen on normal CD4+
Induction of CD69 antigen on normal CD4+ and CD8+ lymphocyte subsets and its relationship with the phenotype of responding T-cells
✍ S. Rutella; C. Rumi; M.B. Lucia; T. Barberi; P.L. Puggioni; M. Lai; A. Romano; R 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 176 KB 👁 2 views

We evaluated phenotype and apoptotic status of normal CD4 ؉ CD69 ؉ and CD8 ؉ CD69 ؉ peripheral blood T-lymphocytes after short-term challenge with escalating concentrations of phytohemagglutinin (PHA). The frequency of CD69-coexpressing CD4 ؉ and CD8 ؉ T-cells and CD69 staining intensity increased f

Enrichment and detection of live antigen
Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion
✍ Helmut Brosterhus; Susanne Brings; Heike Leyendeckers; Rudolf A. Manz; Stefan Mi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 140 KB 👁 1 views

Following appropriate antigen-specific stimulation, CD4 + and CD8 + T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isol

Differential regulation of Ly49 expressi
Differential regulation of Ly49 expression on CD4+ and CD4–CD8– (double negative) NK1.1+ T cells
✍ Markus Sköld; Susanna Cardell 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 196 KB 👁 1 views

The pan-NK cell marker NK1.1, present in some mouse strains, is also found on a subset of TCRalphabeta+ lymphocytes termed NKT cells. These cells are primarily CD4+ or CD4-CD8- (double negative, DN), and both NKT subpopulations contain cells reactive with the MHC class I-like molecule CD1d. Murine N