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Detection and enrichment of antigen-specific CD4+ and CD8+ T cells based on cytokine secretion

✍ Scribed by John D.M Campbell


Book ID
117702304
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
296 KB
Volume
31
Category
Article
ISSN
1046-2023

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Following appropriate antigen-specific stimulation, CD4 + and CD8 + T lymphocytes rapidly express cytokines. Based on this stimulation-induced cytokine secretion and using cell suface affinity matrix technology we have developed a new method that permits specific, rapid and efficient detection, isol