𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selective and sensitive determination of lipoyllysine (protein-bound α-lipoic acid) in biological specimens by high-performance liquid chromatography with fluorescence detection

✍ Scribed by Soichiro Satoh; Masahiro Shindoh; Jun Zhe Min; Toshimasa Toyo’oka; Takeshi Fukushima; Shinsuke Inagaki

Book ID
108047970
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
655 KB
Volume
618
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Rapid Quantitative Determination of Sial
Rapid Quantitative Determination of Sialic Acids in Glycoproteins by High-Performance Liquid Chromatography with a Sensitive Fluorescence Detection
✍ K.R. Anumula 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 563 KB

Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co