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Highly sensitive determination of free fatty acids in human serum by high-performance liquid chromatography with fluorescence detection

✍ Scribed by Masatoshi Yamaguchi; Reiko Matsunaga; Shuuji Hara; Masaru Nakamura; Yosuke Ohkura

Book ID
108449620
Publisher
Elsevier Science
Year
1986
Weight
640 KB
Volume
375
Category
Article
ISSN
0378-4347

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Simple and highly sensitive determinatio
Simple and highly sensitive determination of free fatty acids in human serum by high performance liquid chromatography with fluorescence detection
✍ Tetsuharu Iwata; Kouji Inoue; Masaru Nakamura; Masatoshi Yamaguchi πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 391 KB πŸ‘ 2 views

## Abstract A highly sensitive and simple reversed phase high performance liquid chromatographic (HPLC) method for the quantitative determination of free fatty acids in human serum is presented. The method is based on the direct derivatization of serum fatty acids with 6,7‐dimethoxy‐1‐methyl‐2(1__H

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Determination of free fatty acids (FFAs) in control serum and human plasma was carried out by highperformance liquid chromatography with electrochemical detection. Peak height for palmitic, palmitoleic, stearic, oleic, linoleic, and arachidonic acids at a detection potential of ؊415 mV vs a saturate