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Secretion expression of recombinant glucagon inEscherichia coli

โœ Scribed by Chongwei Wen; Zuoren Wang; Peng Du; Renbao Gan; Shangquan Zhu

Publisher
Science in China Press (SCP)
Year
2001
Tongue
English
Weight
545 KB
Volume
44
Category
Article
ISSN
1674-7305

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High-level expression and secretion of streptokinase inEscherichia coli
โœ Jae Hyeong Ko; Do Keun Park; Il Chul Kim; Si Hyoung Lee; Si Myung Byun ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 364 KB

The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 ml of LB-a