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Recombinant expression of mouse osteocalcin protein inEscherichia coli

โœ Scribed by Ji-Hyun Kim; Soonok Park; Hae-Won Kim; Jun-Hyeog Jang


Publisher
Springer Netherlands
Year
2007
Tongue
English
Weight
205 KB
Volume
29
Category
Article
ISSN
0141-5492

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The effects of process conditions on proteolysis of three recombinant IgG-binding proteins ZT3,ZZT3 and protein A in E. coli were studied. SDS/PAGE shows that the relative amount of degradation intermediates of ZT3 in a shake flask cultivation is much higher than that in a bioreactor culture. The ra