Scanning isoelectric focusing of cholera enterotoxin in polyacrylamide gels
β Scribed by W. W. Yotis; N. Catsimpoolas; F. Montiel
- Book ID
- 110557693
- Publisher
- Springer Netherlands
- Year
- 1975
- Tongue
- English
- Weight
- 697 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0003-6072
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## Abstract Isoelectric focusing in 60 cm long gels improves resolution in multipleβcomponent systems by providing very shallow, linear pH gradients. Broad range ampholytes available commercially or synthesized in our laboratory develop pH gradients of 0.1 pH/cm at voltages of 1500 to 4500 V, with
The flexibility and usefulness of polyacrylamide gel-slab isoelectric focusing is greatly increased by performing separations with glass slides embedded in the gel. After separation, each gel-coated slide can be treated separately; as many as eight individual reactions can be observed after a single