Sandwich enzyme immunoassay of total insulin in the serum containing insulin antibodies
โ Scribed by Masato Tominaga; Mamoru Honda; Yasuo Itoh; Osamu Mokuda; Tadasu Ikeda; Hiroto Mashiba
- Book ID
- 115825942
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 67 KB
- Volume
- 169
- Category
- Article
- ISSN
- 0009-8981
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A highly sensitive enzyme immunoassay who had been treated with insulin for 0.6of anti-insulin antibodies in human serum 24 months. The detection limit of anti-inis described. Serum samples were sub-sulin IgG in human serum was 1,000 to jected to successive processes of the in-3,000-fold less than t
A novel enzyme immunoassay of anti-insulin IgG in human serum is described. A serum sample containing anti-insulin IgG was treated with dextran-charcoal at pH 6.0 to remove endogenous insulin and subsequently incubated with dinitrophenyl biotinyl nonspecific rabbit IgG-insulin conjugate. The reactio