Enzyme immunoassay of total insulin in human serum containing anti-insulin antibodies
โ Scribed by Takeyuki Kohno; Eiji Ishikawa; Satoru Sugiyama; Syuji Nakamura; Masaki Kamano; Takafumi Ichioka; Katsuyuki Nakajima
- Book ID
- 115825593
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 464 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0009-8981
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A highly sensitive enzyme immunoassay who had been treated with insulin for 0.6of anti-insulin antibodies in human serum 24 months. The detection limit of anti-inis described. Serum samples were sub-sulin IgG in human serum was 1,000 to jected to successive processes of the in-3,000-fold less than t
A novel enzyme immunoassay of anti-insulin IgG in human serum is described. A serum sample containing anti-insulin IgG was treated with dextran-charcoal at pH 6.0 to remove endogenous insulin and subsequently incubated with dinitrophenyl biotinyl nonspecific rabbit IgG-insulin conjugate. The reactio