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Sample preparation methods for MALDI-MS profiling of bacteria

✍ Scribed by Ondrej Šedo; Ivo Sedláček; Zbyněk Zdráhal

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
707 KB
Volume
30
Category
Article
ISSN
0277-7037

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✦ Synopsis

Abstract

Direct matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few‐minute analytical procedure providing information extending up to sub‐species level underlines the potential of the MALDI‐MS profiling in comparison with other methods employed in the field. However, the quality of MALDI‐MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI‐MS profiling methodology. Approaches improving the quality of the MALDI‐MS profiles and universal feasibility of the method are discussed. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:417–434, 2011

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