Non-specific, on-probe cleanup methods for MALDI-MS samples
✍ Scribed by Yingda Xu; Merlin L. Bruening; J. Throck Watson
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 242 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0277-7037
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
| I. | Introduction | 429 |
| II. | Decontamination with Films of Commercial Polymers | 431 |
| | A. Polyvinylidenedifluoride and Nitrocellulose | 431 |
| | B. Nylon | 431 |
| | C. Nafion | 432 |
| | D. Polyethylene (PE) and Polypropylene (PP) | 432 |
| | E. Polyurethane | 432 |
| | F. Paraffin and Teflon | 432 |
| | G. Ion‐Exchange Materials | 433 |
| III. | Decontamination with Thin Layers of Matrix Crystals | 433 |
| | A. Mechanism of Adsorption to Matrix Crystals | 434 |
| | B. Preparation of Desalting Matrix Crystals | 434 |
| | 1. Thin Layers of Matrix Crystals | 434 |
| | 2. Hydrophilic Spots of Matrix in Hydrophobic Polymer Layers | 435 |
| IV. | Decontamination with SAMs and Ultrathin Polymer Films | 435 |
| | A. Decontamination with Hydrophobic Interactions | 435 |
| | B. Decontamination with Electrostatic Interactions | 436 |
| | C. Patterned SAM/Polymer Films | 437 |
| V. | Conclusions | 438 |
| Acknowledgments | | 438 |
| References | | 438 |
High concentrations of contaminants such as salts and surfactants are often present in biological samples to solubilize or stabilize analytes such as proteins. Unfortunately, the presence of those contaminants often precludes direct analysis by MALDI‐MS. Selective adsorption of analytes directly on modified MALDI probes, followed by rinsing to remove contaminants, overcomes this problem. This review focuses on various modifications of MALDI probes to allow the adsorption of proteins and DNA, even in a large excess of salt or surfactant. Interfaces deposited on the MALDI probes to adsorb analytes include films of commercial polymers, thin layers of matrix crystals, self‐assembled monolayers, and ultrathin polymer films. Hydrophobic and ionic interactions both effect analyte adsorption on those interfaces, and patterned interfaces allow the concentration and purification of analyte molecules. © 2003 Wiley Periodicals, Inc., Mass Spec Rev 22:429–440, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mas.10064