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Salting out of aqueous proteins: Phase equilibria and intermolecular potentials

✍ Scribed by C. J. Coen; H. W. Blanch; J. M. Prausnitz


Publisher
American Institute of Chemical Engineers
Year
1995
Tongue
English
Weight
897 KB
Volume
41
Category
Article
ISSN
0001-1541

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An aqueous two-phase system containing 7% Dextran T 500-5% polyethylene glycol (PEG) 6000 has been adopted for rapid selective stepwise extractions of high-mobility-group proteins and histones from both isolated chromatin and intact nuclei of calf thymus. After the dissociated proteins in the PEG ph