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Routine determination of unconjugated 3-methoxy-4-hydroxyphenylglycol in plasma using high-performance liquid chromatography with electrochemical detection

โœ Scribed by Stephen G. Molyneux; Michael Franklin


Book ID
108449699
Publisher
Elsevier Science
Year
1985
Weight
445 KB
Volume
341
Category
Article
ISSN
0378-4347

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A new method utilizing high-performance liquid chromatography with electrochemical detection for the determination of unconjugated 3-methoxy-4-hydroxyphenylglycol, a major metabolite of norepinephrine, in human plasma is described. A novel internal standard, 3-ethoxy-4-hydroxyphenylglycol, was used

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We herein report the simultaneous determination of the levels of noradrenaline (NA), and 3-methoxy-4-hydroxyphenylglycol (MHPG), a major metabolite of NA. The sample was subjected to a Sep Pak C18 cartridge prior to the NA and MHPG assay by high-performance liquid chromatography with an electrochemi