Rous sarcoma virus production in mixed cultures of mammalian rous sarcoma cells and chick embryo cells

✍ Scribed by N. Yamaguchi; M. Takeuchi; T. Yamamoto

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: French
• Weight: 900 KB
• Volume: 4
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910040514

✦ Synopsis

The induction of Rous sarcoma virus f RS V ] production was analyzed in mi-ved cultures of mouse or hamster Rous sarcoma cells and chick embryo cells treated with ultraviolet inactivated hemagglutinating virus of Japan ( U V-H V J ) . The pre-treatment of UV-HVJ with a certain concentration of anti-HVJ serum increased the yield of infectious R S V in mixed cultures enabling a quantitative analysis of this phenomenon to be made. R S V-inducing ability of a mixture of H VJ and anti-H VJ serum was affected by the concentration of anti-HVJ serum and showed changes in parallel with the cell fuJion activity of the mixture. The effect of UV-H VJ on R S V induction was not achieved by incubation of the cell mixture at 0" C as it was after shaking at 37" C. These findings suggest that the production of infectious R S V in mixed cultures may be initiated in heterokaryons of mammalian Rous sarcoma cells and chick embryo cells. The number of infective centers in mixed cultures was, however, as sniall as 1 % of that of heterokaryons. It is possible that only a sniall jruction of the heterokaryon population is productive of infectious RSV.