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A change in growth potential of cells after conversion by Rous sarcoma virus

✍ Scribed by John P. Bader

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
593 KB
Volume
70
Category
Article
ISSN
0021-9541

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✦ Synopsis

Chick embryo cells transformed by Rous sarcoma virus (RSV) were able to grow in suspension, either as colonies when trapped in nutrient agar, or in spinner cultures using liquid medium. TWO strains of RSV, RSV (RAV-1) and Schmidt-Ruppin RSV, were able to increase the ability of chick embryo cells to grow in suspension but Rous-associated virus (RAV-1) and polyoma virus were not. Cells growing in suspension supported high levels of RSV production and a simple method for propagating large amounts of virus is suggested. Suspended noninfected cells, which do not grow extensively, lose their ability to be infected by RSV, suggesting that cellular divisions must be in progress for successful infection by RSV.

Methods

Abbreviations. RSV, Rous sarcoma virus, unless otherwise denoted is strain RSV

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