✦ LIBER ✦

Inhibition of development of transformation by rous Sarcoma virus in cultures of high cell density

✍ Scribed by Joseph M. Clark; John P. Bader

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 625 KB
Volume: 83
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040830206

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✦ Synopsis

Abstract

The effect of cell density on morphological transformation of chick embryo cells by Rous Sarcoma Virus (RSV) was examined in this study, and a cell density optimum for transformation was found. Less than 10% of the transformed foci appearing at the optimum density (2.5 × 10^4^ cells per cm^2^) developed at high cell densities, and the diameters of the foci (an indication of the number of cells per focus) decreased with increasing cell density. No correlation was found between the decrease in transformation at high cell densities and the effect of cell density on the initial rate of cell proliferation, although dissociation of transformation from incorporation of radioactive precursors into nucleic acids could not be established. Redistribution of cells infected at high density showed that only a small proportion of successfully infected cells developed into foci. The results indicate that transformation of cells containing the RSV genome can be suppressed by physiological factors accompanying high cell density.

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