Room temperature phosphorescence optosensor for tetracyclines

✍ Scribed by Fausto Alava-Moreno; Marta Elena Díaz-García; Alfredo Sanz-Medel

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 648 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85025-f

✦ Synopsis

A flow-through optosensor for tetracyclines based on the tetracycline-europium chelate room temperature phosphorescence energy transfer is proposed. The sensor is developed in conjunction with ti flow-injection analysis system and is based on the transient immobilization on a non-ionic resin (packed in a flow-through cell) of the tetracycline-europium chelate. 'The analytical performance characteristics of the proposed sensor for semiautomated analysis and control of very low levels of tetracycline were as follows: the detection limits for tetracycline, oxytetracycline and chlortetracycline were 0.25,0.30 and 0.40 ng ml-', respectively, with a relative standard deviation of 1% for determination of 0.24 pg ml-' of each antibiotic (n = 10). Most of the common metal ions in biological samples did not interfere, except Fe(M) which caused serious interference and should be masked with l,lOphenanthrollne. The recommended method has been successfully tested for determination of tetracyclines in clinical samples (urine and pharmaceutical preparations).