Room temperature phosphorescence properties of dansyl chloride and its derivatives

✍ Scribed by Long-Di Li; Wei-Guo Huang

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 490 KB
• Volume: 312
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00212-i

✦ Synopsis

Emission conditions and spectral properties of 5-dimethylaminonaphthalene sulfonylchloride (DNS-Cl) for micelle stabilized room temperature phosphorescence (MS-RTP) and solid substrate room temperature phosphorescence (SS-RTP) are studied. In sodium dodecyl sulphate micelle systems, MS-RTP emission of DNS-Cl can be induced with TINO, as heavy atom perturber and Na2S0, for deoxygenation, A,,/&,, = 328/558 nm. The intensity of RTP is linear with the concentration of DNS-Cl in the range of 1 X lop7 to 4 X 10e5 mol I-'. On several solid substrates, the SS-RTP of DNS-Cl can be achieved with lead acetate (Pb(AC),) as heavy atom perturber. On filter paper and for A,,/&,, = 335/575 nm, the linear range is 12 to 300 pmol DNS-Cl in 0.3 ~1 sample volume and the limit of detection is 12 pmol. On the other hand, the MS-RTP emission of derivative products for amino acid and ethanolamine can also be induced. Compared with that of DNS-Cl, not only the h,,/A,, of the derivative products show a clear shift to longer wavelengths, but also the optimal pH increased from 7 to 10. These results show that DNS-Cl can be used not only as a fluorescence reagent, but also for RTP.