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Role of Sulfhydryl Groups in Firefly Luciferase *

โœ Scribed by DeLuca, M.; Wirtz, G. W.; McElroy, W. D.


Book ID
126850489
Publisher
American Chemical Society
Year
1964
Tongue
English
Weight
631 KB
Volume
3
Category
Article
ISSN
0006-2960

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Secretion of firefly luciferase inE.coli
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A DNA segment carrying the full-length, intronless firefly luciferase gene was inserted into the high expression secretion vector, pIN-Wr -ompA. Upon induction of gene expression, luciferase activity was detected in extracts prepared from periplasmic fractions. The results indicated that the OmpA s