Opiate Analgesics' Dual Role in Firefly Luciferase Activity
โ Scribed by Sudhaharan, T.; Reddy, A. Ram
- Book ID
- 126883831
- Publisher
- American Chemical Society
- Year
- 1998
- Tongue
- English
- Weight
- 178 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0006-2960
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The continuous bioluminescent assay of ATP has been adapted t o the study of Mg\*+-dependent ATPases, including the "a+; K+) pump, in amphibian tissues. A discrete bioluminescent assay procedure for ATPase has also been developed. Components of the firefly luciferase assay reagent modify the observ
## Abstract Firefly luciferase (EC 1.13.12.5) (FL) is the key enzyme in the firefly bioluminescence method (FB), which is widely used to determine the viability of living cells. The FB method can also be applied to monitoring the influence of different pollutants, such as pesticides. Firefly lucife