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Role of activated oxygen species on the mutagenicity of benzo[a]pyrene

✍ Scribed by C. E. Wei; K. Allen; H. P. Misra

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
424 KB
Volume
9
Category
Article
ISSN
0260-437X

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✦ Synopsis

Different scavengers of active oxygen species (superoxide dismutase, catalase, mannitol and dimethylfuran) were tested in the Ames Salmonella assay to determine the role of the reactive oxygen species in the benzo[a]pyrene (B[alP) mutagenesis process. Exogenously added superoxide dismutase or catalase at 1lL100 pg ml-' top agar, or S 1 2 mM mannitol showed no effect on B[a]P mutagenicity in the presence of S9 mix. However, dimethylfuran (DMF), a singlet oxygen scavenger, inhibited in a dose-related manner the mutagenic response of B[a]P in the presence of the microsomal fraction. DMF at 3 and 6 mM inhibited the number of revertants by 69 and 93% for strain TA 100, and 76 and 78% for TA 98, respectively. DMF at these levels was neither toxic nor mutagenic to the bacteria. The result indicates that singlet oxygen may play an important role in promoting B[a]P mutagenicity.

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