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The effect of paraquat on the mutagenicity of benzo(a)pyrene

✍ Scribed by C. I. Wei; K. Allen; H. P. Misra

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 453 KB
Volume: 5
Category: Article
ISSN: 0260-437X
DOI: 10.1002/jat.2550050510

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✦ Synopsis

Elevated activities of superoxide dismutase (SOD) were detected in histidine-requiring strains of Salmonella typhimurium after the bacteria were-preincubated for 1 h at 37OC with S-9 mix and paraquat (methylviologen, PQ") at 1 0 -4 M . A fivefold increase in SOD level was found for strains TA 9 8 and TA 100. These elevated levels of SOD activity were correlated with a significant reduction of the mutagenicity of metabolically activated benzo(a)pyrene (B(a)P) in these tester bacteria when evaluated in a preincubation assay system. A 69.0-92.5% and 23.5-66.9% reduction was noticed when 0.5-4.0 pg per plate of B(a)P was used in TA 98 and TA 100, respectively. However, exogenous superoxide dismutase at 10-100 pg ml-' added to top agar had no significant effect on the number of revertants produced by activated B(a)P. These data indicate a major role of intracellular superoxide anion in promoting mutagenicity of B(a)P.

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