S. typhimurium strain BY324 is temperature sensitive due to a mutation (rpo C32) in the gene for the RNA polymerase fi' subunit. Transcription of T7 DNA by RNA polymerase purified from this strain is temperature sensitive in vitro. The enzyme is slightly defective in template binding and RNA chain i
โฆ LIBER โฆ
RNA polymerase II-dependent plant in vitro transcription systems
โ Scribed by Qun Zhu
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 337 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0960-7412
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We have used surface plasmon resonance (SPR) to follow variations in the concentrations of binary complexes as RNA polymerase moves into a transcriptionally competent initiation complex with immobilized DNA fibres containing promoter sequences. The use of SPR to follow complex binding phenomena is d