✦ LIBER ✦

Real time in vitro analysis of transcription by RNA polymerase on immobilized DNA fibres

✍ Scribed by Iain K. Pemberton; Malcolm Buckle

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 107 KB
Volume: 12
Category: Article
ISSN: 0952-3499
DOI: 10.1002/(sici)1099-1352(199909/10)12:5<322::aid-jmr471>3.0.co;2-w

No coin nor oath required. For personal study only.

✦ Synopsis

We have used surface plasmon resonance (SPR) to follow variations in the concentrations of binary complexes as RNA polymerase moves into a transcriptionally competent initiation complex with immobilized DNA fibres containing promoter sequences. The use of SPR to follow complex binding phenomena is described. We have also followed the changes in the mass of initiation complexes following addition of the nucleotide triphosphates prerequisite for transcription on the immobilized template. These signals are interpreted in terms of the escape of RNA polymerase into elongation mode and the subsequent synthesis of nascent RNA molecules.

📜 SIMILAR VOLUMES

Quantitative analysis of estrogen recept

Quantitative analysis of estrogen receptor-α and -β messenger RNA expression in breast carcinoma by real-time polymerase chain reaction

✍ Kyoko Iwao; Yasuo Miyoshi; Chiyomi Egawa; Noriko Ikeda; Fumine Tsukamoto; Shinza 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 134 KB

## Background: Estrogen action is mediated not only through a classic estrogen receptor (er) (er-alpha) but also through a second er (er-beta) that has a structure and function similar to er-alpha. a correlation between er-beta mrna expression with er and progesterone receptor (pr) protein levels a