𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Multiple effects of an RNA polymerase β' mutation on in vitro transcription

✍ Scribed by Young, Barbara S. ;Wright, Andrew

Publisher
Springer
Year
1977
Tongue
English
Weight
585 KB
Volume
155
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

S. typhimurium strain BY324 is temperature sensitive due to a mutation (rpo C32) in the gene for the RNA polymerase fi' subunit. Transcription of T7 DNA by RNA polymerase purified from this strain is temperature sensitive in vitro. The enzyme is slightly defective in template binding and RNA chain initiation, but the major defect is in RNA chain elongation. The rate of RNA chain elongation is reduced 4 5 fold relative to wild-type. RNA chain termination does not appear to be affected by the fi' mutation. While the elongation defect is suppressed by glycerol or dimethylsulfoxide, the initiation defect is not. Possible roles for the fi' subunit in enzyme function are discussed in light of these results.

📜 SIMILAR VOLUMES

Effects of DNA methylation on specific t
Effects of DNA methylation on specific transcription by RNA polymerase IIin vitro
✍ P. L. Molloy 📂 Article 📅 1986 🏛 Springer 🌐 English ⚖ 421 KB

In vitro transcription in a HeLa cell lysate by RNA polymerase II directed by a chicken feather keratin gene promotor has been studied using unmethylated template DNA and DNA methylated in vitro by HpalI methylase. The efficiency of specific gene transcription from methylated DNA was dependent on to

Real time in vitro analysis of transcrip
Real time in vitro analysis of transcription by RNA polymerase on immobilized DNA fibres
✍ Iain K. Pemberton; Malcolm Buckle 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 107 KB 👁 2 views

We have used surface plasmon resonance (SPR) to follow variations in the concentrations of binary complexes as RNA polymerase moves into a transcriptionally competent initiation complex with immobilized DNA fibres containing promoter sequences. The use of SPR to follow complex binding phenomena is d

The effect of rifampicin upon the transc
The effect of rifampicin upon the transcription of RNA polymerase β-gene in Escherichia coli
✍ Bass, I. A. ;Danilevskaya, O. N. ;Mekhedov, S. L. ;Fedoseeva, V. B. ;Gorlenko, Z 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 728 KB

We studied the rate of synthesis of beta-and beta'-subunits of DNA-dependent RNA polymerase and the rate of beta-polypeptide mRNA synthesis in rifampicin-treated bacteria. The chosen antibiotic doses did not significantly inhibit the total RNA and protein synthesis in rifampicin-sensitive bacteria.

Effect of mutations in the RNA polymeras
Effect of mutations in the RNA polymerase gene and that of the transcription termination factor rho on expression of the Escherichia coli galactose operon with an IS2 polar insertion
✍ Yarulin, V. R. ;Gorlenko, Zh. M. 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 270 KB

We analysed the effects on the expression of the gal operon of six phenotypically different mutations in the Escherichia coli RNA polymerase genes in combination with wild-type, rho, and mutant, rho15, alleles of the gene for the transcription termination factor. RNA polymerase mutations can enhance