RNA Methodologies || The Northern Blot
β Scribed by Farrell, Robert E.
- Book ID
- 121243566
- Publisher
- Elsevier
- Year
- 1993
- Weight
- 985 KB
- Category
- Article
- ISBN
- 0122497007
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This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. *RNA Methodologies 4e* presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA
We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (G