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Northern blotting: transfer of denatured RNA to membranes

Book ID
109937762
Publisher
Nature Publishing Group
Year
2005
Tongue
English
Weight
36 KB
Volume
2
Category
Article
ISSN
1552-4450

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We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (G