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RNA extraction from virus-diseased sweet potato for reverse transcriptase polymerase chain reaction analysis

✍ Scribed by Nick S. Fenby; Susan Seal; Richard W. Gibson; Gary D. Foster


Book ID
110671137
Publisher
Humana Press Inc
Year
1998
Tongue
English
Weight
363 KB
Volume
10
Category
Article
ISSN
1073-6085

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## Abstract A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 ΞΌl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties o