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Heparin interference with reverse transcriptase polymerase chain reaction of RNA extracted from lungs after ischemia-reperfusion

✍ Scribed by Xiao-hui Bai; Stefan Fischer; Shaf Keshavjee; Mingyao Liu


Book ID
110915798
Publisher
Springer
Year
2000
Tongue
English
Weight
666 KB
Volume
13
Category
Article
ISSN
0934-0874

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## Abstract A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties o