A single gene from Streptomyces tenjimariensis, conferring resistance to kanamycin, apramycin and sisomicin, has been cloned in Streptomyces lividans. The mechanism of resistance involves methylation of 16S RNA in the 30S ribosomal subunit.
Ribosomal resistance of an istamycin producer, Streptomyces tenjimariensis, to aminoglycoside antibiotics
β Scribed by Hirokazu Yamamoto; Kunimoto Hotta; Yoshiro Okami; Hamao Umezawa
- Book ID
- 118316111
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 332 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0006-291X
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Resistance to aminoglycoside antibiotics in Micromonospora purpurea (the producer of gentamicin C complex), Streptomyces tenebrarius (the nebramycin producer) and Streptomyces tenjimariensis (which makes istamycin) occurs at the level of the ribosome. Reconstitution analysis has revealed, in each ca
When DNA fragments from Micromonospora purpurea (the producer of gentamicin) were cloned in Streptomyces lividans, a gentamicin-resistant strain was obtained in which the ribosomes were highly resistant both to gentamicin and to kanamycin. Reconstitution analysis revealed that such resistance result