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Reversible heat inactivation of cypridina luciferase

✍ Scribed by Chase, Aurin M.

Publisher: Wiley (John Wiley & Sons)
Year: 1946
Tongue: English
Weight: 177 KB
Volume: 27
Category: Article
ISSN: 0095-9898
DOI: 10.1002/jcp.1030270207

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A bioluminescent enzyme immunoassay using estriol labeled with reversibly inactivated bacterial luciferase is described. An estriol derivative bearing an alkylthiolsulfonate is linked to the cysteinyl thiols of Iuciferase by formation of mixed disuhide linkages; thus, luciferase becomes inactive. Af