Purification of Cypridina luciferase
โ Scribed by McElroy, William D. ;Chase, Aurin M.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1951
- Tongue
- English
- Weight
- 419 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0095-9898
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Doiita at tlie & f : ~r i i i ( ~ 13iologic:tl Td:il)or:itoiy, U'ootls Holc, Mass. My thanks arc ~l n e t o Rfr. Rolwrt Ilydc, in ch:irgr of tlic ceiitrifugta, who riot only opmatrcl the np1i:iratiiq but also contri1)ntcd mnrh lielpf111 :itid 1" nrticd ndvicc.
Denaturation o f proteins by urea has received a great deal of attention. Some of the earlier studies are cited in the paper by Steinhardt ('38) and in the general review of protein denaturation by Neurath et al. ( '44). The papers of Kanzmann et al. ('53) contain numerous references to more recent
## Abstract Prostaglandin E~2~ is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E~2~ utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E~2~ amount could be quantified over the concentration r