## Abstract Oocyte ribosomes stimulate H^3^–5–uridine incorporation into RNA when microinjected into the vegetal cells of early cleavage embryos. Preparations with a low percentage of monosomes, such as those of swimming larvae and adult frog liver, give little stimulation. E. __coli__ ribosomes fa
Reversible delay of normal development of frog embryos by inhibition of DNA synthesis
✍ Scribed by Flickinger, R. A.
- Publisher
- John Wiley and Sons
- Year
- 1966
- Tongue
- English
- Weight
- 634 KB
- Volume
- 161
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Demembranated gastrulae that had been cultured in 0.05% cytosine arabinoside for a day and subsequently cultured in a saline medium, experienced a delay (1-2 days) in the time at which flexure of the tail-buds first occurred. Histological sections showed these embryos to be essentially normal. Isotopic experiments
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## Abstract Staged wildtype embryos __Drosophila melanogaster__ were permeabilized and then subjected to a short pulse of either methyl‐^3^H‐thymidine, one of four different inhibitors of DNA synthesis (mitomycin C, 5‐fluorouracil, nalidixic acid, or 1‐β‐D‐arabino‐furanosylcytosine‐5′‐monophosphate
## Abstract Mouse embryos were treated with 5‐bromodeoxyuridine (BrdUrd) for 24 hours at various preimplantation stages to determine its effect on early postimplantation development. The inhibitory effect of BrdUrd (10^−7^ to 10^−6^ M) on trophoblast outgrowth and inner cell mass (ICM) development
Actinomycin D (0.008 pg/gm of body weight) injected intraperitoneally every two hours, produced a prompt 50% inhibition of RNA synthesis in the jejunum of mice, and a delayed inhibition of DNA synthesis, that reached its maximum inhibition (68% of control values) 4.5 hours after the first injection
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