✦ LIBER ✦

Stimulation of RNA synthesis in developing frog embryos by microinjection of ribosomes

✍ Scribed by Rollins, J. W. ;Flickinger, R. A.

Publisher: John Wiley and Sons
Year: 1973
Tongue: English
Weight: 511 KB
Volume: 183
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401830207

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Oocyte ribosomes stimulate H^3^–5–uridine incorporation into RNA when microinjected into the vegetal cells of early cleavage embryos. Preparations with a low percentage of monosomes, such as those of swimming larvae and adult frog liver, give little stimulation. E. coli ribosomes fail to affect RNA synthesis. Short term labeling experiments at the gastrula stage in which only nuclear RNA was labeled suggest that added ribosomes may increase nuclear transcription and not cytoplasmic conservation of D‐RNA in polysomes.

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