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Reversed-phase liquid chromatographic resolution of amino acid enantiomers by derivatization with 2,3,4,6-tetra-O-acetyl-β-d-glucopyranosyl isothiocyanate

✍ Scribed by Noriyuki Nimura; Haruo Ogura; Toshio Kinoshita

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
359 KB
Volume
202
Category
Article
ISSN
1873-3778

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✦ Synopsis

A novel method for reversed-phase high-performance liquid chromatographic resolution of amino acid enantiomers by the formation of diastereomers using 8 new chiral reagent, 2,3,4,6-tetra-O-acetyl+D-glucopyranosyl isothiocyanate (GITC), is described. GITC reacts readily with enantiomeric amino acids at room temperature and the reaction mixture can directly be injected into the chromatograph. The derivatives were detected spectrophotometrically at 250 run. Complete resolutions were observed for all enantiomers examined on a reversed-phase column eluted with aqueous methanol.

📜 SIMILAR VOLUMES

Resolution of optical isomers of 2,3,4,6
Resolution of optical isomers of 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate (GITC)–derivatized DL-amino acids by micellar electrokinetic chromatography
✍ Hiroyuki Nishi; Tsukasa Fukuyama; Masaaki Matsuo 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 475 KB

## Abstract Resolution of isomers of 2,3,4,6‐tetra‐__O__‐acetyl‐β‐D‐glucopyranosyl isothiocyanate (GITC)–derivatized DL‐amino acids was accomplished using micellar electrokinetic chromatography with sodium dodecyl sulfate (SDS) solutions. These solutes were not separated by the usual capillary zone