Reversed-phase ion-pair partition chromatography of carboxylates and sulphonates
β Scribed by Karl-Gustav Wahlund
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 813 KB
- Volume
- 115
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
A reversed-phase partition chrematographic system for separation of organic ations as ion pairs with qnatemary ammonium ions has been developed. Commercia!, hydrophobized silica supports are used wi& I-pentanol as st&ianzy phase and aqueocs solutions of tetrabutylammoGum (TBA) = mobile phase. The sepzxxtian of
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Some possibilities are described to improve the separation between catecholamines and their corresponding metanephrines in reversed-phase ion-pair partition chromatography. The retention of amines can be influenced by the perchlorate concentration of the mobile phase, while the retention of acidic m
## Abstract The results of investigations with ion pair chromatography on RP~2~, RP~8~ and RP~18~ thin layers are described. Heptane sulphonic acid is used as ion pair former. The best results are achieved on RP~18~βlayers with the counter ion in the mobile phase.
The parameters that influence the retention and selectivity of glucuronides in reversed-phase ion-pair liquid chromatography were investigated. The extent of ion-pair retention was dependent on the degree of substitution of the ammonium counter ion used and increased in the order 4-< 2-< 3-< l-subs