𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Reversed-phase ion-pair liquid chromatography of glucuronides : Retention and selectivity

✍ Scribed by Morgan Stefansson; Douglas Westerlund

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
664 KB
Volume
499
Category
Article
ISSN
1873-3778

No coin nor oath required. For personal study only.

✦ Synopsis

The parameters that influence the retention and selectivity of glucuronides in reversed-phase ion-pair liquid chromatography were investigated.

The extent of ion-pair retention was dependent on the degree of substitution of the ammonium counter ion used and increased in the order 4-< 2-< 3-< l-substituted, comparing counter ions with the same number of carbon atoms. The retentions and selectivities were influenced by the type of buffer system, solid phase and organic modifier used. The selectivities obtained were so high that a urine sample spiked with four glucuronides could be analysed by a direct injection technique and UV detection (254 nm).

πŸ“œ SIMILAR VOLUMES

Selective retention of catecholamines an
Selective retention of catecholamines and their derivatives reversed-phase ion-pair partition chromatography
✍ J. De Jong; C.F.M. van Valkenburg; U.R. Tjaden πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 720 KB

Some possibilities are described to improve the separation between catecholamines and their corresponding metanephrines in reversed-phase ion-pair partition chromatography. The retention of amines can be influenced by the perchlorate concentration of the mobile phase, while the retention of acidic m

Ion pair chromatography on reversed-phas
Ion pair chromatography on reversed-phase layers
✍ Volkmann, D. πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 English βš– 203 KB πŸ‘ 1 views

## Abstract The results of investigations with ion pair chromatography on RP~2~, RP~8~ and RP~18~ thin layers are described. Heptane sulphonic acid is used as ion pair former. The best results are achieved on RP~18~‐layers with the counter ion in the mobile phase.