Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents
โ Scribed by Hancock, W.; Bishop, C.; Prestidge, R.; Harding, D.; Hearn, M.
- Book ID
- 123617068
- Publisher
- American Association for the Advancement of Science
- Year
- 1978
- Tongue
- English
- Weight
- 678 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0036-8075
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๐ SIMILAR VOLUMES
This report describes the use of hydrophobic ion-pairing reagents in the rapid analysis of peptides by reversed-phase high-pressure liquid chromatography. It was found that combination of a hydrophobic anion such as hexanesulphonate with the c&ionic groups (R&H,) of a peptide resulted in a decreased
Compounds IVb-IVg were prepared similarly from I11 and the appropriate alcohols (Table I). p-Chlorophenyl Benzo[ b]-3-methyl-2-furancarbamate (1Vb)-A solution of I11 (2.01 g, 0.01 mole) and p-chlorophenol(l.28 g, 0.01 mole) in 30 ml of dry benzene was refluxed for 4 hr. The solvent was evaporated,