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Reverse zymography using fluorogenic substrates for protease inhibitor detection

โœ Scribed by Quang T. Le; Atsushi Ohashi; Sayu Hirose; Nobuhiko Katunuma

Book ID
102184745
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
399 KB
Volume
26
Category
Article
ISSN
0173-0835

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Several new fluorogenic substrates capable of measuring the proteolytic activities of pancreatic elastase, trypsin, and trypsinlike enzymes were prepared. The fluorophore utilized in these substrates is 6-aminoquinoline (6-AQ), a leaving group that affords significant improvement over the use of oth